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bt301 midterm papers 2014

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bt301 midterm papers 2014

bt301 midterm past paper 1 2018

Bt 301
Xray difrction 5mrks
Electroporation 5
Minor and major groove length and nucleotides 3
Gene 2
Mcq
1...tranfers phosphate from atp
2..... Has single chromosome with protein core
3. Dna has one oh removed at .... Carbon
4 Pcr is used for ....Dna rna dna$rna protein
5 .... Amplifies the number in vitro
Pcr hybridization clonning
6 Hae 3 is identifies .... Nucleotides
7 Ti is found in ......
8 Claudia wala aya tha k new ka nam kya tha
9 Making of rna from dna is called .....
10 Yeast genome was sequenced till.....
11 Nomenclature was presented by.......
12 Dna is lysed by..... Enzymes
13 Psi angle of a helix is.....

bt301 midterm past paper 2 2017

BT301:- 12 pm

Q:1 what is autoradiography?

Q:2 what is the role of electrophoration?

Q:3 What is the role of Ori in plasmids?

Q:4 Differentiate between multimer,ologimer and protomer

Q:5 Explain Chargaff rule

Q:6 what is nuclein?

Join VU Group

bt301 midterm past paper 3 2017

Define central dogma 2
Effects of Ethidium bromide on body.3
Chargaff rules. 5
Electroporation. 5
Promoter 2

: Autoradiography
2: Multimer
3: differentiate between nucleotides and nucleosidesâ€‹

bonding between parts of a nucleotide. (2)

Q2: What is Selfish DNA? (2)

Q3: Define Green biotechnology and give one example. (3)

Q4: Briefy describe Type-III R-M. (3)

Q5: Define Biotechnology and just give the name of its various branches. (5)
6- explain briefly structures of protein.
What is the central dogma of molecular biology? 2
Relaxed plasmid? 2
Benefit of genomic in madicine research? 3
Western blotting? 3
Grifth experiment? 5
electrophoration technique? 5
what is pseudo gene?
write 5 standard amino acids name.
write about electroporation role.
write different structures of proteins.
write one differential point between super coiled and relaxed loops? long question
1.Biotechnology define and write its branches
2.Electroporation technique
Q6: Explain Restriction Modification Phenomenon. (5)

4: Importance of origin region in plasmids

bt301 midterm past paper 4 2017

4:Euchromatic & hetrchromatin (2)
5:Ntural plasmid (2)
6:Principle of wetern blotting
1:define protiemics (2)
2: defind genome (2)
3:palindromic sequence (3)
4:name of any five amino acids (3)
5: work of frinklins and wilkins (5)
6: R-M system mechanism (5)
Q:1 what is autoradiography?
Q:2 what is the role of electrophoration?
Q:3 What is the role of Ori in plasmids?
Q:4 Differentiate between multimer,ologimer and protomer
Q:5 Explain Chargaff rule
Q:6 what is nuclein? write different structures of proteins.
write one differential point between super coiled and relaxed loops? long question
1.Biotechnology define and write its branches
2.Electroporation technique
What factor affect the DNA mobility in electrophoresis gel?
Major minor groove its length and how many nucleotides are present?
What type of cell can be transformed using liposomes?
Restrictions modification phenomenon
Biotechnonolgy and its branches
Side effect of ethidium bromide
Prgancy test Grifth experiment? 5
electrophoration technique? 5
1-Differentiate b/w protomers, oligomers and multimers
2-What is electroporation technique

2-What is electroporation technique
3-Importance of Ori region in plasmid
4- Retrotranspos in humans
5-Wht r 3 units for the formation of nucleotide.

bt301 midterm past paper 5 2017

: Chagraf rule
2: Restriction modification phenomenon
Short questions
1: Autoradiography
2: Multimer
3: differentiate between nucleotides and nucleosides
4: Importance of origin region in plasmidsTop of Form1chirg aff rule (5)
2: R-M system mechanism (5)
3: major and minor groove , number of nucleotide in them (3)
4:Euchromatic & hetrchromatin (2)
5:Ntural plasmid (2)
6:Principle of wetern blotting
1:define protiemics (2)
2: defind genome (2)
3:palindromic sequence (3)
4:name of any five amino acids (3)
5: work of frinklins and wilkins (5)
6: R-M system mechanism (5)
Q:1 what is autoradiography?
Q:2 what is the role of electrophoration?
Q:3 What is the role of Ori in plasmids?
Q:4 Differentiate between multimer,ologimer and protomer
Q:5 Explain Chargaff rule
Q:6 what is nuclein? write different structures of proteins.
write one differential point between super coiled and relaxed loops? long question
1.Biotechnology define and write its branches

It is concerned with the discovery and development of innovative drugs and treatments. ? Red biotechnology

It is used in lyses (protease K)

Nuclien discovered??

LTR consists of …. Bp?

Benefits of genomic research???

Nucleotide differ due to change in??? bases

The hydrophilicbackbones ofalternating deoxyribose and phosphate groups are on the outside of the double helix, facing the surrounding water.

How many bases DNA and RNA have???

mRNA transcribe the template into Protein

How many functional groups attach to alpha carbon including alcohol R group.

Capsid protects the Nucleic acid in virus.

The bacterial chromosome is avery long (up to 1mm).

Genomics, in contrast, is the studof the entirety of an organism’s genes –called the genome.

PCR amplification is used to amplify?

Yeast genome was completelysequenced by 1996

LINES • 1-5 kb

Koi restriction enzyme walamcqthabaki 3 ydnaiL
2*2

1- Major technique use for genome characterization

2- Reverse transcriptase
3*2

1- Nomenclature for restriction enzyme

2- Benefits of genomic research?
5*2

1- What is biotechnology? Write its field name

2- Function of retrotransposons in human genome.

bt301 midterm past paper 6 2017

Bt301
Justify influence virus in one line ?
RNA types ?
Explain chargaff rule ??
Explain restriction modification??
Northern blotting??

My today paper of BT301
Total Q 26 (40 marks)
20 MCQS
4 Short Q and 2 long Q
Short Q:
1. What is meant by Nicking of DNA? (2 marks)
2. What is restortranspons? (2)
3. What is difference between Protomer, Oligomer and Multimer? (3 marks)
4. Define the principle of western Blotting? (3)
2 long Q:
1. Write down contribution of franklin and Wilkins? (5 marks)
2. Note on Restriction-Modification principle?

bt301 midterm past paper 7 2017

BT301

What was pregnancy test performed to test gender in ancient period?
What is the role of transposons in human genome?
What was the Frederick grifth experiment about genetic material?
What is the role of electriporation technique?
What are the general consideration for nomenclature for restricted enzyme.? Give example
What is the main components consisting the RNA molecule.?

bt301 midterm past paper 8 2017

Bt301
1-Define autoradiography?
2-Define traditional biotechnology?
3-What are protomer, oligomer and multimer?
4-Explain the contribution of Franklin and wilkins?
5-Write a note on restriction-modofication system?
6-Define palindromic sequence?

bt301 midterm past paper 9 2017

BT301 current paper
10.30 am Sunday 18-12-16
20 mcqs
5*2=10 q
2*2=4 q
3*2=6 q
MCQS:-
It is concerned with the discovery and development of innovative drugs and treatments. ? Red biotechnology
It is used in lyses (protease K)
Nuclien discovered??
LTR consists of …. Bp?
Benefits of genomic research???
Nucleotide differ due to change in??? bases
The hydrophilic backbones of alternating deoxyribose and phosphate groups are on the outside of the double helix, facing the surrounding water.
How many bases DNA and RNA have???
mRNA transcribe the template into Protein
How many functional groups attach to alpha carbon including alcohol R group.
Capsid protects the Nucleic acid in virus.
The bacterial chromosome is avery long (up to 1mm).
Genomics, in contrast, is the stud of the entirety of an organism’s genes –called the genome.
PCR amplification is used to amplify?
Yeast genome was completely sequenced by 1996
LINES • 1-5 kb
Koi restriction enzyme walamcqthabaki 3 ydnaiL
2*2
1- Major technique use for genome characterization
2- Reverse transcriptase
3*2
1- Nomenclature for restriction enzyme
2- Benefits of genomic research?
5*2
1- What is biotechnology? Write its field name
2- Function of retrotransposons in human genome.